Two hundred years of Czech double bass coincides with the 200th anniversary of the Prague Conservatoire, where from the very beginning double bass had its place. We present six of the large number of outstanding double bass players. They all were either graduates of Prague Conservatoire or students of the musicians who took their professional training there. Already in 1809, Václav House (Wenzel Hause), the first professor of the Prague Conservatoire, published his Textbook His methodology based on careful study of double bass technique was taken over and further developed by his followers. He correctly perceived the need to adapt double bass. from the technical and acoustic point of view. to the newly emerging romantic style. In contrast to the formerly used tuning (e. g. three cord Italian double bass tuned in fifths or classical four-string double bass with the possibility to descend to C below the E) he started using four-string instrument tuned in perfect fourths. In the third volume of his textbook Schule des Virtuosen published 1844 in Prague, he advises the soloists to use the new fingering. Especially he stresses the need for the so called lower bow grasp as opposed to the Italian method, when the bow is held in the same way as with the violin or violoncello. In 1837 an excellent soloist and teacher Josef Hrabě graduated in the class of Václav House. He became another double bass professor and finished already started reforms (fingerboard division into pitches and semipitches). He trained the whole school of young musicians, who were able to introduce new methodology into many European countries